The Green Alternative for Decontamination of Biosafety Cabinets, Incubators and More!

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

This paper describes how to use a custom manufactured, commercially available enclosed cell culture system for basic and preclinical research. Biosafety cabinets (BSCs) and incubators have long been the standard for culturing and expanding cell lines for basic and preclinical research. However, as the focus of many stem cell laboratories shifts from basic research to clinical translation, addit...

Microbial Decontamination of Spices Using Cold Plasma

Most of spices are produced using traditional systems. In unsanitary conditions, spices can contain large numbers of pathogenic microbes such as bacteria, molds and yeasts. Some microorganisms are known as human pathogens, which need disinfection mechanisms that minimize their potential harms to active substances in spices. Use of contaminated spices in foods can significantly decrease the shel...

Efficacy of dry mist of hydrogen peroxide (DMHP) against Mycobacterium tuberculosis and use of DMHP for routine decontamination of biosafety level 3 laboratories.

Mycobacterium tuberculosis is a major cause of morbidity and mortality worldwide and is becoming a greater concern due to the development of multidrug-resistant strains. M. tuberculosis can contaminate rooms, medical equipment, and research laboratories and has the propensity to be highly resistant to decontamination. The aim of this study was to determine the efficacy of room disinfection with...

The Biological Weapons Convention: From International Obligations to Effective National Action

The objective of this study was to evaluate both condensing (wet) and non-condensing (dry) vaporous hydrogen peroxide (VHP) technologies for decontaminating a worst-case scenario Class II biosafety cabinet. A 23-year-old Class II, type A2 biosafety cabinet equipped with loaded HEPA filters and an inoperable blower was used for this study. Biological indicators were placed at various locations w...

Appraisal of the practical effectiveness of biosafety controls in biotechnology with special reference to safety cabinets